In vitro steroidogenic effects of mixtures of persistent organic pollutants (POPs) extracted from burbot (Lota lota) caught in two Norwegian lakes
Identifieur interne : 000369 ( Main/Exploration ); précédent : 000368; suivant : 000370In vitro steroidogenic effects of mixtures of persistent organic pollutants (POPs) extracted from burbot (Lota lota) caught in two Norwegian lakes
Auteurs : Karin E. Zimmer [Norvège] ; Mauricio Montano [Luxembourg (pays)] ; Ingrid Olsaker [Norvège] ; Ellen Dahl [Norvège] ; Vidar Berg [Norvège] ; Camilla Karlsson [Norvège] ; Albertinka J. Murk [Pays-Bas] ; Janneche U. Skaare [Norvège] ; Erik Ropstad [Norvège] ; Steven Verhaegen [Norvège]Source :
- Science of the total environment [ 0048-9697 ] ; 2011.
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- Pascal (Inist)
- Wicri :
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Abstract
This study investigated the effects of two mixtures of persistent organic pollutants (POPs) on steroidogenesis in the H295R cell line. The two mixtures were obtained from the livers of burbot (Lota lota) caught in two Norwegian lakes (Mjøsa and Losna) with different contaminant profiles. Steroid hormone levels in the cell culture medium and mRNA levels of 16 genes involved in steroidogenesis were investigated. The crude Lake Mjøsa extract had to be diluted ten times more than the Lake Losna extract in order to prevent cytotoxicity. The ten times diluted Lake Mjøsa mixture had higher levels of DDT and derivates (Σ DDTs, 1.7 times) and brominated flame retardants (Σ BDEs and HBCD, 15-25 times) than the Lake Losna mixture, which, on the other hand, had higher concentrations of Σ PCBs (1.5 times higher) and also of HCB, Σ HCH isomers and Σ chlordane isomers (5-20 times higher). In the cell culture media, only cortisol levels were increased at the highest exposure concentration to the Lake Mjøsa mixture, while both cortisol and estradiol levels were increased following exposure to the two highest Lake Losna mixture exposure concentrations. Testosterone levels decreased only at the highest exposure concentration of the Lake Losna mixture. Multivariate models suggested that Σ PCBs, and to a lesser extent Σ DDTs, were responsible for the cortisol responses, while estradiol and testosterone alterations were best explained by HCB and Σ PCBs, respectively. Exposure to the mixtures generally increased mRNA levels, with smaller effects exerted by the Lake Mjøsa mixture than the Lake Losna mixture. It was concluded that both mixtures affected steroidogenesis in the H295R cells. Small differences in mixture composition, rather than the high content of brominated flame retardants in the Lake Mjøsa mixture, were suggested to be the most probable reason for the apparent differences in potencies of the two mixtures.
Affiliations:
- Luxembourg (pays), Norvège, Pays-Bas
- Gueldre (province), Østlandet
- Oslo, Wageningue
- Université de Wageningue
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Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Montano, Mauricio" sort="Montano, Mauricio" uniqKey="Montano M" first="Mauricio" last="Montano">Mauricio Montano</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Environment and Agro-biotechnologies, Centre de Recherche Public-Gabriel Lippmann, 41, rue de Brill</s1><s2>4422, Belvaux</s2><s3>LUX</s3><sZ>2 aut.</sZ></inist:fA14><country>Luxembourg (pays)</country><wicri:noRegion>4422, Belvaux</wicri:noRegion></affiliation></author><author><name sortKey="Olsaker, Ingrid" sort="Olsaker, Ingrid" uniqKey="Olsaker I" first="Ingrid" last="Olsaker">Ingrid Olsaker</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Dahl, Ellen" sort="Dahl, Ellen" uniqKey="Dahl E" first="Ellen" last="Dahl">Ellen Dahl</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Production Animal Clinical Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Berg, Vidar" sort="Berg, Vidar" uniqKey="Berg V" first="Vidar" last="Berg">Vidar Berg</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>5 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Karlsson, Camilla" sort="Karlsson, Camilla" uniqKey="Karlsson C" first="Camilla" last="Karlsson">Camilla Karlsson</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Production Animal Clinical Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Murk, Albertinka J" sort="Murk, Albertinka J" uniqKey="Murk A" first="Albertinka J." last="Murk">Albertinka J. Murk</name><affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Wageningen University, Section of Toxicology, P.O. Box, 6700 EA</s1><s2>Wageningen</s2><s3>NLD</s3><sZ>7 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>Wageningen</wicri:noRegion><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Wageningen-IMARES, P.O. Box 68, 1970 AB</s1><s2>IJmuiden</s2><s3>NLD</s3><sZ>7 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>IJmuiden</wicri:noRegion></affiliation></author><author><name sortKey="Skaare, Janneche U" sort="Skaare, Janneche U" uniqKey="Skaare J" first="Janneche U." last="Skaare">Janneche U. Skaare</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>5 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>National Veterinary Institute of Norway, P.O. Box 750</s1><s2>0106 Oslo</s2><s3>NOR</s3><sZ>8 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Ropstad, Erik" sort="Ropstad, Erik" uniqKey="Ropstad E" first="Erik" last="Ropstad">Erik Ropstad</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Production Animal Clinical Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Verhaegen, Steven" sort="Verhaegen, Steven" uniqKey="Verhaegen S" first="Steven" last="Verhaegen">Steven Verhaegen</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Production Animal Clinical Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">12-0117644</idno><date when="2011">2011</date><idno type="stanalyst">PASCAL 12-0117644 INIST</idno><idno type="RBID">Pascal:12-0117644</idno><idno type="wicri:Area/PascalFrancis/Corpus">000019</idno><idno type="wicri:Area/PascalFrancis/Curation">000099</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000022</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000022</idno><idno type="wicri:doubleKey">0048-9697:2011:Zimmer K:in:vitro:steroidogenic</idno><idno type="wicri:Area/Main/Merge">000372</idno><idno type="wicri:Area/Main/Curation">000369</idno><idno type="wicri:Area/Main/Exploration">000369</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">In vitro steroidogenic effects of mixtures of persistent organic pollutants (POPs) extracted from burbot (Lota lota) caught in two Norwegian lakes</title><author><name sortKey="Zimmer, Karin E" sort="Zimmer, Karin E" uniqKey="Zimmer K" first="Karin E." last="Zimmer">Karin E. Zimmer</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Montano, Mauricio" sort="Montano, Mauricio" uniqKey="Montano M" first="Mauricio" last="Montano">Mauricio Montano</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Environment and Agro-biotechnologies, Centre de Recherche Public-Gabriel Lippmann, 41, rue de Brill</s1><s2>4422, Belvaux</s2><s3>LUX</s3><sZ>2 aut.</sZ></inist:fA14><country>Luxembourg (pays)</country><wicri:noRegion>4422, Belvaux</wicri:noRegion></affiliation></author><author><name sortKey="Olsaker, Ingrid" sort="Olsaker, Ingrid" uniqKey="Olsaker I" first="Ingrid" last="Olsaker">Ingrid Olsaker</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Dahl, Ellen" sort="Dahl, Ellen" uniqKey="Dahl E" first="Ellen" last="Dahl">Ellen Dahl</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Production Animal Clinical Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Berg, Vidar" sort="Berg, Vidar" uniqKey="Berg V" first="Vidar" last="Berg">Vidar Berg</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>5 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Karlsson, Camilla" sort="Karlsson, Camilla" uniqKey="Karlsson C" first="Camilla" last="Karlsson">Camilla Karlsson</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Production Animal Clinical Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Murk, Albertinka J" sort="Murk, Albertinka J" uniqKey="Murk A" first="Albertinka J." last="Murk">Albertinka J. Murk</name><affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Wageningen University, Section of Toxicology, P.O. Box, 6700 EA</s1><s2>Wageningen</s2><s3>NLD</s3><sZ>7 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>Wageningen</wicri:noRegion><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Wageningen-IMARES, P.O. Box 68, 1970 AB</s1><s2>IJmuiden</s2><s3>NLD</s3><sZ>7 aut.</sZ></inist:fA14><country>Pays-Bas</country><wicri:noRegion>IJmuiden</wicri:noRegion></affiliation></author><author><name sortKey="Skaare, Janneche U" sort="Skaare, Janneche U" uniqKey="Skaare J" first="Janneche U." last="Skaare">Janneche U. Skaare</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>5 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>National Veterinary Institute of Norway, P.O. Box 750</s1><s2>0106 Oslo</s2><s3>NOR</s3><sZ>8 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Ropstad, Erik" sort="Ropstad, Erik" uniqKey="Ropstad E" first="Erik" last="Ropstad">Erik Ropstad</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Production Animal Clinical Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Verhaegen, Steven" sort="Verhaegen, Steven" uniqKey="Verhaegen S" first="Steven" last="Verhaegen">Steven Verhaegen</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Production Animal Clinical Sciences, Norwegian School of Veterinary Science, P.O. Box 8146 Dep</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Science of the total environment</title><title level="j" type="abbreviated">Sci. total environ.</title><idno type="ISSN">0048-9697</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Science of the total environment</title><title level="j" type="abbreviated">Sci. total environ.</title><idno type="ISSN">0048-9697</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological activity</term><term>Hormone</term><term>In vitro</term><term>Lakes</term><term>Lota lota</term><term>Mixture</term><term>Norway</term><term>Persistent organic pollutant</term><term>Steroidogenesis</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Mélange</term><term>Polluant organique persistant</term><term>Activité biologique</term><term>Hormone</term><term>Stéroïdogenèse</term><term>In vitro</term><term>Lac</term><term>Norvège</term><term>Lota lota</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Norvège</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Hormone</term><term>Lac</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study investigated the effects of two mixtures of persistent organic pollutants (POPs) on steroidogenesis in the H295R cell line. The two mixtures were obtained from the livers of burbot (Lota lota) caught in two Norwegian lakes (Mjøsa and Losna) with different contaminant profiles. Steroid hormone levels in the cell culture medium and mRNA levels of 16 genes involved in steroidogenesis were investigated. The crude Lake Mjøsa extract had to be diluted ten times more than the Lake Losna extract in order to prevent cytotoxicity. The ten times diluted Lake Mjøsa mixture had higher levels of DDT and derivates (Σ DDTs, 1.7 times) and brominated flame retardants (Σ BDEs and HBCD, 15-25 times) than the Lake Losna mixture, which, on the other hand, had higher concentrations of Σ PCBs (1.5 times higher) and also of HCB, Σ HCH isomers and Σ chlordane isomers (5-20 times higher). In the cell culture media, only cortisol levels were increased at the highest exposure concentration to the Lake Mjøsa mixture, while both cortisol and estradiol levels were increased following exposure to the two highest Lake Losna mixture exposure concentrations. Testosterone levels decreased only at the highest exposure concentration of the Lake Losna mixture. Multivariate models suggested that Σ PCBs, and to a lesser extent Σ DDTs, were responsible for the cortisol responses, while estradiol and testosterone alterations were best explained by HCB and Σ PCBs, respectively. Exposure to the mixtures generally increased mRNA levels, with smaller effects exerted by the Lake Mjøsa mixture than the Lake Losna mixture. It was concluded that both mixtures affected steroidogenesis in the H295R cells. Small differences in mixture composition, rather than the high content of brominated flame retardants in the Lake Mjøsa mixture, were suggested to be the most probable reason for the apparent differences in potencies of the two mixtures.</div></front></TEI><affiliations><list><country><li>Luxembourg (pays)</li><li>Norvège</li><li>Pays-Bas</li></country><region><li>Gueldre (province)</li><li>Østlandet</li></region><settlement><li>Oslo</li><li>Wageningue</li></settlement><orgName><li>Université de Wageningue</li></orgName></list><tree><country name="Norvège"><region name="Østlandet"><name sortKey="Zimmer, Karin E" sort="Zimmer, Karin E" uniqKey="Zimmer K" first="Karin E." last="Zimmer">Karin E. Zimmer</name></region><name sortKey="Berg, Vidar" sort="Berg, Vidar" uniqKey="Berg V" first="Vidar" last="Berg">Vidar Berg</name><name sortKey="Dahl, Ellen" sort="Dahl, Ellen" uniqKey="Dahl E" first="Ellen" last="Dahl">Ellen Dahl</name><name sortKey="Karlsson, Camilla" sort="Karlsson, Camilla" uniqKey="Karlsson C" first="Camilla" last="Karlsson">Camilla Karlsson</name><name sortKey="Olsaker, Ingrid" sort="Olsaker, Ingrid" uniqKey="Olsaker I" first="Ingrid" last="Olsaker">Ingrid Olsaker</name><name sortKey="Ropstad, Erik" sort="Ropstad, Erik" uniqKey="Ropstad E" first="Erik" last="Ropstad">Erik Ropstad</name><name sortKey="Skaare, Janneche U" sort="Skaare, Janneche U" uniqKey="Skaare J" first="Janneche U." last="Skaare">Janneche U. Skaare</name><name sortKey="Skaare, Janneche U" sort="Skaare, Janneche U" uniqKey="Skaare J" first="Janneche U." last="Skaare">Janneche U. Skaare</name><name sortKey="Verhaegen, Steven" sort="Verhaegen, Steven" uniqKey="Verhaegen S" first="Steven" last="Verhaegen">Steven Verhaegen</name></country><country name="Luxembourg (pays)"><noRegion><name sortKey="Montano, Mauricio" sort="Montano, Mauricio" uniqKey="Montano M" first="Mauricio" last="Montano">Mauricio Montano</name></noRegion></country><country name="Pays-Bas"><region name="Gueldre (province)"><name sortKey="Murk, Albertinka J" sort="Murk, Albertinka J" uniqKey="Murk A" first="Albertinka J." last="Murk">Albertinka J. Murk</name></region><name sortKey="Murk, Albertinka J" sort="Murk, Albertinka J" uniqKey="Murk A" first="Albertinka J." last="Murk">Albertinka J. Murk</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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